-New York City, US, Central Park (Start)
-New York City(John F. Kennedy International Airport) to Johannesburg, South Africa(Johannesburg International Airport)
-Johannesburg (Lanseria Airport)
-Livingstone, Zambia (Livingstone Airport)
-Livingstone (Victoria Falls -Knife's Edge)
-Livingstone (Boiling Pot, Zambezi River)
-Livingstone (Batoka Gorge) (Air or Land)
-Songwe Village (unaired)
Spoilers:
In the first Fast Forward of the race, teams had to hike down a steep slope to Boiling Pot. Rob and Brennan were first to arrive, so they received it. The Detour for this leg was Air or Land. In Air, teams had to take a zipline and then bunjee jump down the side of the gorge. In Land, teams had to take a long hike down the mountains. All teams (except Rob & Brennan) chose Air. The Roadblock, which was unaired (but available on the DVD set), was to eat an ostrich egg.